NGS-Based On-/Off-Target Analysis

Target Enrichment Sequencing (TES)

TES is an NGS-based, time and cost-effective approach to investigate regions of interest in the genome. GeneWerk has streamlined this approach for on-/off-target analysis for gene editing safety assessment.

Capture predicted and non-predicted genomic alterations

GeneWerk’s TES approach for on-/off-target analysis is based on the selective capture of a target sequence using biotinylated RNA ‘baits’ homologous to the genomic sequence of interest.

Apart from donor and vector sequence-specific RNA baits, we also include pre-designed RNA baits targeting in-silico predicted genomic areas.

This helps us to capture and analyze not only thousands of predicted off-target events but also indels, translocations and non-predicted off-target alterations close to these predicted sites.

Interrogate all possible on-/off-events in a single reaction

  • Our highly streamlined approach captures all possible on-/off-target events, in the same reaction in an unbiased and quantitative manner.
  • Gather deep insights into the nature and specificity of your genome editing approach:
  • Quantify non-homologous end joining (NHEJ) at on- and off-target sites
  • Assess unintended on- and off-target modifications
  • Detect and quantify translocation events
  • Detect non-predicted off-target sites

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