On-/Off-Target Analysis

Genome editing using designer nucleases such as CRISPR/Cas9, ZFNs and TALENs allow the targeted introduction of genetic material into a specific locus of the mammalian genome. However, undesired on- and off-target genome modifications may arise which could cause genomic instability and disrupt the functionality of otherwise normal genes potentially causing safety concerns in pre-clinical and clinical studies

Genome editing

Current off-target analysis techniques rely mainly on bioinformatics predictions and genome-wide off-target detection techniques. Such predictions lack reliability as they cover only a small portion of potential genomic alterations. And off-target mutations with a frequency below 0.5% remain mostly undetected by current genome-wide off-target detection techniques. We are the pioneer to develop unbiased assays for genome-wide on-/off-target analysis.

Targeted genome editing – delineating desired from undesired outcomes 

At GeneWerk, we offer comprehensive PCR and NGS-based assays for gene editing safety assessment. Our versatile and cost-effective assays interrogate both on- and off-target genomic alterations.

Our state-of-the-art assays in combination with powerful in-house bioinformatics pipelines reliably quantify the ratios between the desired targeted integration and non-desired outcomes such as translocations and indels.


At GeneWerk, we adapted the Shearing Extension Primer Tag Selection Ligation-Mediated PCR (S-EPTS/LM PCR) method to analyze on-target and off-target integrations arising from genome editing.

  • Convenient detection of on-/off-target alterations and random integrations
  • Adaptable to address specific questions raised by regulatory authorities
  • Customized bioinformatics analysis
Read more about PCR


At GeneWerk, we use target enrichment sequencing (TES) for genome-wide detection and quantification of on- and off-target alterations. Our time and cost-effective approach captures all possible on-/off-target events in a single reaction.


  • NGS-based approach – avoid PCR artifacts
  • Captures predicted as well as non-predicted on-/off-target events
  • Customized bioinformatics analysis
Read more about NGS

Want to get in touch?

Consult with our experts about solutions to your biosafety challenges.

Contact us

Latest news

Read our latest press releases and announcements below.

A Sad Message From Our Team. Loss…

We mourn the loss of our dear friend, mentor, eminent scientist, co-founder and CEO...

Read More

Protagen Protein Services merges with GeneWerk

Protagen Protein Services (PPS) today announced that it will merge with GeneWerk, an Ampersand...

Read More

GeneWerk Announces Growth Equity Investment from Ampersand…

GeneWerk will expand capabilities to meet rapidly growing demand for cell and gene therapy...

Read More

Insight into Spatial and Temporal Trends of…

Genewerk GmbH in collaboration with University Heidelberg Germany provided an insight into spatial and...

Read More
I agree to the Privacy Policy and Terms of Service.